When you first walk in, hit wait to get queens to start moving to left. From there, move left to get to where you are on top of the third queen so they won't move to right. I think if you wait till they start to lay an egg, then move to hit top orb. You will have enough time so the queens will then run to the bottom right corner and you can easily kill them. You'll probably want to have your sword positioned to top left prior to getting in the room. Rest is just clean up.

On a side note, if you hit Control then a key, it will do the action and then the immediate opposite action. Will help out a little so you don't have to hit Q and W or up and down so often.